Plate assay for detection of Leptospira interrogans serovar pomona hemolysin.
نویسندگان
چکیده
A plate assay which utilizes the addition of a blood agar overlay for detection of hemolysin by colonies of Leptospira interrogans serovar pomona is described.
منابع مشابه
Copurification of Leptospira interrogans serovar pomona hemolysin and sphingomyelinase C.
The hemolytic and sphingomyelinase C activities of supernatants of cultures of Leptospira interrogans serovar pomona tended to copurify when isoelectric fractionation was carried out. Both activities focused primarily at pH 8.1. Considered in conjunction with other circumstantial evidence, the results led to the conclusion that sphingomyelinase C is responsible for hemolysis.
متن کاملCompetitive enzyme-linked immunosorbent assay for detection of Leptospira interrogans serovar pomona antibodies in bovine sera.
A competitive enzyme-linked immunosorbent assay (ELISA) using a specific monoclonal antibody (M898) was developed for detection of bovine antibodies to Leptospira interrogans serovar pomona. This assay was evaluated using field sera (n = 190) with serovar pomona microscopic agglutination test (MAT) titers of > or =100 as the positive population (group A); field sera (n = 1,445) which were negat...
متن کاملMonoclonal antibodies reacting with serogroup and serovar specific epitopes on different lipopolysaccharide subunits of Leptospira interrogans serovar pomona.
Monoclonal antibodies with two kinds of specificities, produced against Leptospira interrogans serovar pomona, were studied by agglutination and immunoblotting. Antibodies reacted either exclusively with serovar pomona or with all members of the Pomona serogroup, but none of the antibodies reacted with representative serovars of other serogroups. Both antibodies recognized epitopes on purified ...
متن کاملFirst Genome Sequence of Leptospira interrogans Serovar Pomona, Isolated from a Bovine Abortion
Leptospirosis is a widespread zoonosis and a re-emergent disease of global distribution with major relevance in veterinary production. Here, we report the whole-genome sequence of Leptospira interrogans serovar Pomona strain AKRFB, isolated from a bovine abortion during a leptospirosis outbreak in Argentina.
متن کاملVariable nucleotide tandem-repeat analysis revealing a unique group of Leptospira interrogans serovar Pomona isolates associated with California sea lions.
Leptospira interrogans serovar Pomona isolates were compared by variable nucleotide tandem-repeat typing. Most cattle isolates grouped together, while isolates from pigs and wildlife were distributed across several groups. Significantly, California sea lion isolates formed a unique group, providing evidence that these animals are maintenance hosts of serovar Pomona.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 10 4 شماره
صفحات -
تاریخ انتشار 1979